Cytokine q-RT-PCR, c-Fos immunoreactivity and open field behaviour data in rats following bladder inflammation

Dataset 1: Cytokine Up-regulation following Acute Bladder InflammationLegend: .csv file containing q-RT-PCR cycle time (CT) for each of the 92 inflammatory mediators and 4 housekeeping genes. Delta CT (dCT) and fold change (FC) values are shown for each. Exclusions are denoted by * by the sample or transcript ID.
Dataset 2: Cytokine Up-regulation following Repeated Bladder InflammationLegend: .csv file containing q-RT-PCR cycle time (CT) for each of the 92 inflammatory mediators and 4 housekeeping genes. Delta CT (dCT) and fold change (FC) values are shown for each. Exclusions are denoted by * by the sample or transcript ID.
Dataset 3: Open Field activity following Acute Bladder InflammationLegend: .zip file containing 15 minute videos of open field behaviour in rats 24hrs after acute bladder inflammation (36). File naming reflects individual animal IDs to maintain blinding. Group allocation data available on request from F1000Research.Each file was edited using Adobe Premiere (Creative Cloud) as follows: • A 10 second ‘lead-in’ period prior to introduction of the animal was edited in, either by trimming from the start of the video to a point 10 seconds before introduction of the animal, or by stretching the footage prior to introduction of the animal to a 10 second duration• The arena was straightened, making it easier for others analysing the dataset as only a single arena template is required during automated analysis e.g. using Ethovision, Noldus Software, The Netherlands• File formats were converted from .mpg to .mp4 to reduce the file size (from ~200MB to ~50MB)
Dataset 4: Open Field activity following Repeated Bladder InflammationLegend: .zip file containing 15 minute videos of open field behaviour in rats 24hrs after (38). File naming reflects individual animal IDs to maintain blinding. Group allocation data available on request from F1000Research.Each file has been edited using Adobe Premiere (Creative Cloud) as follows: • A 10 second ‘lead-in’ period prior to introduction of the animal was edited in, either by trimming from the start of the video to a point 10 seconds before introduction of the animal, or by stretching the footage prior to introduction of the animal to a 10 second duration• The arena was straightened, making it easier for others analysing the dataset as only a single arena template is required during automated analysis e.g. using Ethovision, Noldus Software, The Netherlands• File formats were converted from .mpg to .mp4 to reduce the file size (from ~200MB to ~50MB)

Dataset 5: c-Fos Immunoreactivity in the Central Amygdala following Acute Bladder InflammationLegend: .csv file containing area, count, and density data for c-Fos immunoreactivity in the central amygdala. This data underwent extensive manipulation prior to analysis: density values for each subnuclei were pooled for each rostro-caudal level, and used in combination with individual subnuclei data to calculate overall density values.
Dataset 6: c-Fos Immunoreactivity in the Central Amygdala following Repeated Bladder InflammationLegend: .csv file containing area, count, and density data for c-Fos immunoreactivity in the central amygdala.This data underwent extensive manipulation prior to analysis: density values for each subnuclei were pooled for each rostro-caudal level, and used in combination with individual subnuclei data to calculate overall density values.

数据集1：急性膀胱炎后细胞因子上调
说明：包含92种炎症介质和4种管家基因的q-RT-PCR循环时间（CT）的.csv文件。每个样本或转录本的Delta CT（dCT）和倍数变化（FC）值均以表格形式展示。通过*标记表示排除的数据。
数据集2：重复膀胱炎后细胞因子上调
说明：包含92种炎症介质和4种管家基因的q-RT-PCR循环时间（CT）的.csv文件。每个样本或转录本的Delta CT（dCT）和倍数变化（FC）值均以表格形式展示。通过*标记表示排除的数据。
数据集3：急性膀胱炎后开放场活动
说明：包含急性膀胱炎后24小时大鼠开放场行为的15分钟视频.zip文件（编号36）。视频命名反映个体动物ID以保持盲法。F1000Research可提供分组分配数据。每个文件均使用Adobe Premiere（创意云）进行编辑，具体如下：在动物引入前添加10秒的‘引入前’时间段，通过从视频开头修剪至动物引入前10秒，或者通过拉伸动物引入前的画面至10秒长。对竞技场进行校正，以便在自动化分析（如使用Ethovision、Noldus Software，荷兰）时仅需单个竞技场模板。将文件格式从.mpg转换为.mp4以减小文件大小（从约200MB减少至约50MB）。
数据集4：重复膀胱炎后开放场活动
说明：包含重复膀胱炎后24小时大鼠开放场行为的15分钟视频.zip文件。视频命名反映个体动物ID以保持盲法。F1000Research可提供分组分配数据。每个文件均使用Adobe Premiere（创意云）进行编辑，具体如下：在动物引入前添加10秒的‘引入前’时间段，通过从视频开头修剪至动物引入前10秒，或者通过拉伸动物引入前的画面至10秒长。对竞技场进行校正，以便在自动化分析（如使用Ethovision、Noldus Software，荷兰）时仅需单个竞技场模板。将文件格式从.mpg转换为.mp4以减小文件大小（从约200MB减少至约50MB）。
数据集5：急性膀胱炎后中央杏仁核中c-Fos免疫反应性
说明：包含中央杏仁核中c-Fos免疫反应性的面积、计数和密度数据的.csv文件。该数据在分析前经历了广泛的处理：将每个亚核的密度值按颅尾水平合并，并与个体亚核数据结合以计算总体密度值。
数据集6：重复膀胱炎后中央杏仁核中c-Fos免疫反应性
说明：包含中央杏仁核中c-Fos免疫反应性的面积、计数和密度数据的.csv文件。该数据在分析前经历了广泛的处理：将每个亚核的密度值按颅尾水平合并，并与个体亚核数据结合以计算总体密度值。