CIL:37030, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

This is IMAGE #9 (time point: 8 hour, rapid time lapse)of an image group. It is a projection of the time lapse series captured in IMAGE 8. It shows a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. This time lapse series captures the dendritic arbor of this neuron every 60 seconds for 10 minutes. Over time, the tadpole (and the neuron) drifts across the field. In the series, z-stacks of this neuron were acquired at a 2 hour interval for 8 hours. Afterward, a second time lapse series was acquired at a 60 second interval for 10 minutes. This time series shows dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions. The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.