_Sequencing_diversity_outbred_cross_mice_____

The aim is to sequence the genomes of approximately 250 outbred mice in order to identify translocations and other structural variants. The mice are from the Diversity Outcross (DO), an outbred population descended from eight inbred strains, including three wild-derived strains CAST/EiJ, PWK/PhJ, WSB/EiJ. The project is in collaboration with the Mouse Genomes Project led by Dr Thomas Keane at the Sanger Institute to create de-novo assemblies of inbred strains of mice. The results of the DO analysis will inform the de-novo assemblies by producing independent confirmation of translocations etc segregating in these strains. The data will also be of general use to researchers working with DO mice. The method used to map translocations looks for anomalously-mapped short reads at the junctions of translocations. It treats the number of anomalous reads in each individual and at each locus as a quantitative trait which is mapped in a genome scan. The positions of QTLs indicate locations to where the original locus has been translocated. The analysis of the data will be performed jointly with the Wellcome Trust Centre for Human Genetics, by Richard Mott and Jonathan Flint, and the results shared with the mouse genomes assembly group at the Sanger Institute.