A systematic investigation of key factors of nucleic acid precipitation towards optimized DNA/RNA isolation
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Supplementary Figure
1 - Key influence factors and their corresponding conditions of nucleic acid
precipitation;
Supplementary Table 1
- The average recovery rate and its corresponding standard deviation of each
type of nucleic acid by adding alcohol and salt independently or both;
Supplementary Table 2
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each incubation
condition;
Supplementary Table 3
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each centrifugation
force;
Supplementary Table 4
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each centrifugation
time;
Supplementary Table 5
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each volume ratio of
ethanol or isopropanol to nucleic acid solution;
Supplementary Table 6
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under different conditions of
cations;
Supplementary Table 7
- The average recovery rate and its corresponding standard deviation of each
type of nucleic acid upon addition of yeast tRNA as co-precipitator
(carrier);
Supplementary Table 8
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under different conditions of
carriers;
Supplementary Table 9 - The measuring results of
carries in aqueous solutions by NanoDrop 2000.
补充图1 - 核酸沉淀的关键影响因素及其相应条件;
补充表1 - 通过独立添加酒精和盐或同时添加,各种类型核酸的平均回收率及其对应的标准差;
补充表2 - 在每种孵育条件下,各种类型核酸的平均回收率、对应的标准差及其显著性水平;
补充表3 - 在每种离心力下,各种类型核酸的平均回收率、对应的标准差及其显著性水平;
补充表4 - 在每种离心时间下,各种类型核酸的平均回收率、对应的标准差及其显著性水平;
补充表5 - 在每种乙醇或异丙醇与核酸溶液的体积比下,各种类型核酸的平均回收率、对应的标准差及其显著性水平;
补充表6 - 在不同阳离子条件下,各种类型核酸的平均回收率、对应的标准差及其显著性水平;
补充表7 - 添加酵母tRNA作为共沉淀剂(载体)后,每种类型核酸的平均回收率及其对应的标准差;
补充表8 - 在不同载体条件下,每种类型核酸的平均回收率、对应的标准差及其显著性水平;
补充表9 - 通过NanoDrop 2000测量的水溶液中载体的测量结果。
提供机构:
Taylor & Francis



