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A systematic investigation of key factors of nucleic acid precipitation towards optimized DNA/RNA isolation

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tandf.figshare.com2024-05-15 更新2025-03-22 收录
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资源简介:
Supplementary Figure 1 - Key influence factors and their corresponding conditions of nucleic acid precipitation; Supplementary Table 1 - The average recovery rate and its corresponding standard deviation of each type of nucleic acid by adding alcohol and salt independently or both; Supplementary Table 2 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each incubation condition; Supplementary Table 3 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each centrifugation force; Supplementary Table 4 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each centrifugation time; Supplementary Table 5 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each volume ratio of ethanol or isopropanol to nucleic acid solution; Supplementary Table 6 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under different conditions of cations; Supplementary Table 7 - The average recovery rate and its corresponding standard deviation of each type of nucleic acid upon addition of yeast tRNA as co-precipitator (carrier); Supplementary Table 8 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under different conditions of carriers; Supplementary Table 9 - The measuring results of carries in aqueous solutions by NanoDrop 2000.

补充图1 - 核酸沉淀的关键影响因素及其相应条件; 补充表1 - 通过独立添加酒精和盐或同时添加,各种类型核酸的平均回收率及其对应的标准差; 补充表2 - 在每种孵育条件下,各种类型核酸的平均回收率、对应的标准差及其显著性水平; 补充表3 - 在每种离心力下,各种类型核酸的平均回收率、对应的标准差及其显著性水平; 补充表4 - 在每种离心时间下,各种类型核酸的平均回收率、对应的标准差及其显著性水平; 补充表5 - 在每种乙醇或异丙醇与核酸溶液的体积比下,各种类型核酸的平均回收率、对应的标准差及其显著性水平; 补充表6 - 在不同阳离子条件下,各种类型核酸的平均回收率、对应的标准差及其显著性水平; 补充表7 - 添加酵母tRNA作为共沉淀剂(载体)后,每种类型核酸的平均回收率及其对应的标准差; 补充表8 - 在不同载体条件下,每种类型核酸的平均回收率、对应的标准差及其显著性水平; 补充表9 - 通过NanoDrop 2000测量的水溶液中载体的测量结果。
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