A single high‑zinc activation enhancer can control two genes oriented head-to-head in C. elegans

Supplementary Data for paper article, Divergent Enhancer Function of the High-Zinc Activation Element in C. elegans". Supplementary File 1 contains all qPCR data processing spreadsheet (eg. Organized Data Cd, hizr-1 am286, Zn, etc), GraphPad Prism sheets, and log2 average calculation sheets (for superplot). Corresponding folders to figures: “hizr-1 am286” – Fig 1, “Zn” – Fig 2 & 3, Sup Fig S1, S3, & S5, “Cd” – Sup Fig S2, S4, & S5, “mtl-2”, “srh-308” – Fig 4. Supplementary File 2 contains sequencing files for HZAmT and HZAcF loci. “cdr-1_sequencing.seq” and “mtl-2_sequencing.seq” are Sanger sequencing results for the knockout strains. “mtl-2 gk125” folder contains Sanger sequencing results for gk125 allele verification. Supplementary File 3 contains sequences of mtl-2 homologs from C. breneri, C. briggsae, and C. remanei used for alignment in Fig 2.