Time series of host responses to symbiotic T6SS disruption in an amoeba-bacteria symbiosis

In order to understand host responses to symbiont strains with and without an intact symbiotic type 6 secretion system, we infected Dictyostelium discoideum with wildtype vs. ∆tssH Paraburkholderia bonniea and collected samples in a time series to capture the early stages of symbiotic association. Two host strains of Dictyostelium discoideum amoebas were each infected with wildtype (wt) and ∆tssH (delta) Paraburkholderia bonniea bacteria strain bb433. We collected samples at 90, 360, and 2250 minutes post-infection (mpi). The 2250 mpi sample was intended to represent a longer-term infection and was called the 'positive' sample. Additional samples include uninfected 'negative' controls.