scRNAseq of Human Urothelial Carcinoma

Single-cell RNA sequencing (scRNA-seq) was performed on urothelial carcinoma samples to investigate the association between squamous differentiation and gene expression. Treatment-naive samples, ranging from Low Grade Ta to High Grade T2, were taken at the time of initial transurethral resection of bladder tumor (TURBT), and scRNA-seq was performed. Following pathologic review, squamous differentiation was identified as a tumor feature in one sample, and this was compared against the data generated from scRNA-seq using a gene expression signature of squamous differentiation, which included DSG3. We next looked to see what other genes were associated with squamous histology with a focus on the APOBEC family members. We found that APOBEC3A was expressed at increased levels within cells... (for more see dbGaP study page.)