Intestinal organoid expression data from patients with MASLD-cirrhosis versus non-fibrotic MASLD

This study aimed to establish and characterize an in vitro model of human intestinal organoids isolated from duodenal samples of patients with non-fibrotic MASLD and those with MASLD-cirrhosis. Whole transcriptome analysis and the energetic and redox status of the organoids were assessed to characterize intestinal functional impairment in the context of MASLD. We used microarrays to detail the whole transcriptome dysregulation underlying intestinal dysfunction in organoids isolated from chirrotic versus non-fibrotic MASLD patients. Total RNA was isolated from intestinal organoids derived from duodenal samples of patients with non-fibrotic MASLD and those with MASLD-cirrhosis. High-throughput transcript profiling in organoids from five subjects with non-fibrotic MASLD and five subjects with MASLD-cirrhosis was conducted using microarray methodology (Clariom D Assay, Thermo Fisher Scientific).