PCR amplicon sequencing targeting major bacterial genera from honeybee microbiota on Apis mellifera lineage C Apis mellifera unicolor from La Reunion and Bombus terrestris from Clermont-Ferrand

21 primer couples designed to target 8 major taxa from honeybee microbiota have been tested out for specificity by amplicon sequencing via Nanopore MINion technology. Two universal bacterial primers has also been tested (BAC_A and BAC_B). Amplification reactions were made on 3 biological matrix : One extract named "AmelC" was a pool of DNA extracts sampled in France and mostly belonging to the European C lineage. Another sample, named "AmelA", was a pool of 129 DNA extracts from Apis mellifera unicolor workers belonging to the African A lineage and sampled in La Reunion island by Jobart et al., (2024). A third sample, identified as "Bter", was the DNA extracted (DNeasy Blood and Tissue kit, QIAGEN) from four abdomens of Bombus terrestris sampled in France in 2025.