MicroRNA array analysis from infected and uninfected PBMC samples

ABX464, a new drug for curing HIV and treating inflammatory diseases induces upregulation of the anti-inflammatory miR-124. We used microarrays to show the implication of ABX464 in the biogenesis of small noncoding RNAs. So, we decided to evaluate if miRNAs or small nucleolar RNAs (snoRNAs) were differentially regulated by ABX464. We performed a microarray analysis for these RNAs from the PBMCs of 6 donors. Cells that were infected with the YU2 strain, followed by treatment with ABX464 were compared with uninfected and untreated controls. A total of 104 human miRNAs and 40 snoRNAs were significantly differentially expressed in infected PBMCs, when compared to uninfected PBMCs (data file S4), with a false discovery rate lower than 0.05 and fold change higher than 1.5.