AQUACOSM VIMS-Ehux – Core data

The cosmopolitan coccolithophore Emiliania huxleyi is a unicellular alga that forms massive oceanic blooms covering thousands of square kilometers (Tyrrell & Merico 2004). The intricate calcite exoskeleton of E. huxleyi accounts for ~1/3 of total marine CaCO3 production (Monteiro et al. 2016). E. huxleyi blooms are an important source of DMS, which is, by far, the most abundant volatile sulfur compound in the surface ocean and the best studied aerosol precursor (Simó 2001) with a significant climate-regulating role that enhances cloud formation (Alcolombri et al. 2015; Simó 2001). Biotic interactions that regulate the fate of these blooms play a profound role in determining carbon and nutrient cycling in the ocean and feedback to the atmosphere. Annual E. huxleyi spring blooms are frequently terminated following infection by a specific large dsDNA virus (EhV) that belongs to the Coccolithovirus group (Schroeder et al. 2002). Despite the huge ecological importance of host-virus inter..., Mesocosm setup
The mesocosm experiment AQUACOSM VIMS-Ehux was carried out for 24 days between 24th May (day 0) and 16th June (day 23) 2018 in Raunefjorden at the University of Bergen’s Marine Biological Station Espegrend, Norway (60°16′11N; 5°13′07E). The experiment consisted of seven enclosure bags made of transparent polyethylene (11 m3, 4 m deep and 2 m wide, 90% photosynthetically active radiation) mounted on floating frames and moored to a raft in the middle of the fjord. The bags were filled with surrounding fjord water (day -1; pumped from 5 m depth) and continuously mixed by aeration (from day 0 onwards). Each bag was supplemented with nutrients at a nitrogen to phosphorous ratio of 16:1 (1.6 µM NaNO3 and 0.1 µM KH2PO4 final concentration) on days 0-5 and 14-17, whereas on days 6, 7 and 13 only nitrogen was added.
Sampling for flow cytometry analysis
Samples for flow cytometric counts were collected twice a day, in the morning (07:00 AM) and evening (08:00-09:00 PM) from each ba..., FCM data (containing phytoplankton, VLP, and bacteria counts), dissolved inorganic nutrients data, water temperature, salinity data and viral qPCR abundances are available in the Excel files \"AQUACOSM_VIMS-Ehux_CoreData.xlsx\".
All .pdf documents containing the suffix \"_Gates\" represent the different phytoplankton population gates per bag.
NOTE: Version published 28 February 2024 was published to correct units of qPCR analysis of mcp gene from µL to mL.,