Chromosome-scale assembly of the Indianmeal moth <i>Plodia interpunctella</i>

Using the PacBio Sequel II system and SMRT sequencing, we generated high fidelity (HiFi) long-read genomic and transcriptomic sequences for the Indianmeal moth (<i>Plodia interpunctella</i>). The (<i>Plodia interpunctella</i>) genome was highly contiguous (N50 = 9.7 Mbp, L50 = 13) and complete (BUSCO Complete = 99.3%), with complete and contiguous recovery of silk <i>heavy fibroin</i> gene sequences. This study demonstrates that HiFi long-read sequencing can significantly help our understanding of genes with highly contiguous, repetitive regions.本研究借助PacBio Sequel II系统与单分子实时（SMRT）测序技术，为印度谷螟（*Plodia interpunctella*）生成了高保真（HiFi）长读长基因组及转录组序列。该印度谷螟基因组组装连续性优异（N50 = 9.7 Mbp，L50 = 13），完整度极高（BUSCO完整度 = 99.3%），且实现了蚕丝重丝蛋白（heavy fibroin）基因序列的完整且连续组装。本研究证实，HiFi长读长测序可显著助力我们解析具有高度连续、重复区域的基因。