Bacterial transcription factors binding to coding regions regulate the internal cryptic promoters

To analyze the antisense transcripts, we performed LM-seq for Pesudomonas aeruginosa ?algR and Pesudomonas syringae ?rhpS and their corresponding WT strain. Date-minging in ChIP-seq and HT-SELEX results from public dataset, we got the TF binding sites (TFBS) across genome. Coupling analysis with LM-seq and these TFBS, our results revealed that the coding region binding TFs regulate antisense RNA transcription by regulating the activity of cryptic promoters. Overall design: Pesudomonas aeruginosa ?algR and Pesudomonas syringae ?rhpS as well as their corresponding WT strain.