Melting temperatures (<i>T</i>_m)^a of Hum₂₄ and Hum₄₈ G4DNA, ligand bound to pre-formed G4DNA and complexes produced when G4DNA was formed in presence of the indicated ligand at [ligand]:[DNA] ratio ‘r’ = 20 for the monomeric and ‘r’ = 10 for the gemini li

aChanges in the circular dichroism spectral peak (at 295 nm for the G4DNA and the pre-formed G4DNA-ligand complex; at 266 nm for the G4DNA-ligand complex formed in presence of ligand) monitored as a function of temperature using 2 µM strand ODN concentration. bΔTm values were obtained from the difference in the melting temperatures of the ligand bound and uncomplexed G4DNA. For the G4DNA-ligand complexes when G4DNA was formed in presence of the ligand, the solution was first heated to 95°C for 5 min and then cooled slowly. The results are average of two experiments and are within ±0.5°C of each other.