Gobiocypris rarus Transcriptome or Gene expression. Gobiocypris rarus

Changes of testis transcriptome after treatment with 100 ng/L methyltestosterone of Gobio rarus rare. High-throughput sequencing revealed 73,449 unigenes, 1,205 known mature miRNAs, and 939 novel miRNAs in the gonads of G. rarus. For miRNA-seq, 49 (MT25-M vs Con-M), 66 (MT50-M vs Con-M), and 49 (MT100-M vs Con-M) DEMs were identified in the treatment groups. Six mature miRNAs (miR-122-x, miR-574-x, miR-40-y, miR-217-x, lin-4-x, and miR-7-y) as well as seven differentially expressed genes (soat2, inhbb, ihhb, gatm, faxdc2, ebp, and cyp1a1), which may be associated with testicular development, metabolism, apoptosis, and disease response, were assayed using qRT-PCR. Furthermore, miR-122-x (related to lipid metabolism), miR-430-y (embryonic development), miR-217-x and lin-4-x (apoptosis), and miR-7-y (disease) were differentially expressed in the testes of 17MT-exposed G. rarus.