Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient [gene expression]

As organ-specific models of breast cancer bone metastasis do not exist, we established a novel breast cancer line was established from the ER-/PR-/HER2- bone metastasis from a breast cancer patient. This cell line was characterized and compared to the primary tumour and/or normal mammary epithelial cells with regards to phenotypes (i.e. marker expression), gene expression and copy number variation. Total RNA was isolated from the P7731 cell line (3 replicates) and compared to that isolated from the primary tumour (2 replicates).