CNOT6L Couples the Selective Degradation of Maternal Transcriptsto Meiotic Cell Cycle Progression in Mouse Oocyte [total RNA-seq]

Purpose: The goals of this study are to study the function of Cnot6l during oocyte maturation and the differences between loss of Cnot6l and Btg4 during oocyte maturation. Methods:Comparing the degration of transcripts at different stage in WT, Btg4-/- and Cnot6l-/- oocytes by RNA sequencing. Results: Using an optimized data analysis workflow, we mapped about 15 million sequence reads per sample to the mouse genome (build mm9) and identified 23236 transcripts with TopHat workflow. Conclusions: CNOT6L stimulated degradation of maternal transcripts to oocyte meiotic maturation. 2 oocytes at GV ,MI, MII stages and zygotes of WT, Btg4-/- and Cnot6l-/- mice are performed RNA sequencing.研究目的：本研究旨在探究Cnot6l在卵母细胞成熟过程中的功能，以及Cnot6l缺失与Btg4缺失在卵母细胞成熟过程中的差异。 研究方法：通过RNA测序（RNA Sequencing）对比野生型（Wild Type，WT）、Btg4基因敲除型（Btg4-/-）与Cnot6l基因敲除型（Cnot6l-/-）卵母细胞在不同发育阶段的转录本降解情况。 研究结果：采用优化后的数据分析流程，我们将每个样本约1500万条序列读数比对至小鼠基因组（版本mm9），并通过TopHat分析流程鉴定出23236个转录本。 研究结论：CNOT6L可促进母源转录本降解，推动卵母细胞减数分裂成熟。本研究对野生型、Btg4-/-及Cnot6l-/-小鼠的GV期、MI期、MII期卵母细胞及合子各2个样本进行了RNA测序。