Host Factor Experiment (SCL008-R)
收藏DataCite Commons2020-07-23 更新2024-07-13 收录
下载链接：
https://www.viprbrc.org/brc/svExperimentDetails.spg?expAccession=IRD_SV_SCL008-R&decorator=corona&context=1593127042737
下载链接
链接失效反馈官方服务：
资源简介：
Purpose: To obtain samples for transcriptional and proteomic analysis using wild type icSARS CoV and icSARS ExoNI and icSARS dNSP16 mutants in 2B-4 cells/sorted Calu-3 cells with high ACE2 expression. Details: Time Points = 0, 7, 12, 24, 36, 48, 60 and 72h post infection (Note: only 0, 24, 48 and 72hr samples analysed for proteomics); Done in triplicate for RNA and quadruplicate for protein; Replicates are defined as 3 or 4 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection done at an MOI of 5 for wild-type and dNSP16 mutant and an MOI 0f 1 for ExoNI mutant.
提供机构：
Virus Pathogen Resource
创建时间：
2020-06-25
