MIRU-VNTRplus

The geographical origin and the drug susceptibility profile of each strain were stored together with comprehensive genetic lineage information, including the 24-locus MIRU-VNTR profile, the spoligotyping pattern, the single-nucleotide- and large-sequence-polymorphism profiles, and the IS6110 restriction fragment length polymorphism fingerprint. Thanks to flexible import functions, a single or multiple user strains can be analyzed, e.g., for lineage identification with or without the use of reference strains, by best-match or tree-based analyses with single or combined marker data sets. The results can easily be exported. In the present study, we evaluated the database consistency and various analysis parameters both by testing the reference collection against itself and by using an external population-based data set comprising 629 different strains. Under the optimal conditions found, lineage predictions based on typing by 24-locus MIRU-VNTR analysis optionally combined with spoligotyping were verified in >99% of the cases.每株菌株的地理来源与药物敏感性特征，均与完整的遗传谱系信息一并存储，其中包含24位点MIRU-VNTR（24-locus MIRU-VNTR）分型图谱、间隔区寡核苷酸分型（spoligotyping）模式、单核苷酸多态性与大片段多态性分型图谱，以及IS6110限制性片段长度多态性指纹图谱（IS6110 restriction fragment length polymorphism fingerprint）。借助灵活的导入功能，可对单株或多株用户提交的菌株开展分析：例如，可通过单标记或组合标记数据集的最佳匹配分析或基于系统发育树的分析，在是否引入参考菌株的前提下完成谱系鉴定。分析结果可便捷导出。在本研究中，我们通过两种方式评估了数据库一致性与各类分析参数：一是将参考菌株集自身进行交叉验证，二是使用包含629株不同菌株的外部人群源性数据集开展测试。在确定的最优条件下，基于24位点MIRU-VNTR分型（可选择性结合间隔区寡核苷酸分型）的谱系预测准确率超过99%。

搜集汇总

背景与挑战

背景概述

MIRU-VNTRplus是一个用于结核分枝杆菌复合体（MTBC）基因分型的网络数据库和工具，存储了菌株的地理来源、药物敏感性及多种遗传谱系信息（如24-locus MIRU-VNTR、spoligotyping等）。它允许用户导入和分析自身菌株数据，通过相似性搜索或多标记组合进行谱系识别，并支持结果导出，适用于流行病学研究和菌株鉴定。