Human monocyte-derived macrophage response to Aspergillus fumigatus

To investigate the influence of Aspergillus fumigatus on iron regulation in macrophages, we obtained macrophages in culture from human derived monocytes and co-cultured the monocyte-derived macrophages with Aspergillus conidia at a 1:1 ratio. We collected samples at 0, 2, 4, 6 and 8 hours and extracted RNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of control macrophages and macrophage co-cultured with Aspergillus fumigatus at five time points. Comparative gene expression profiling analysis of RNA-seq data for control human monocyte-derived macrophage and human monocyte-derived macrophage co-cultured with Aspergillus fumigatus.