Preclinical development of brain permeable ERß agonist for the treatment of glioblastoma

We examined the mechanisms by estrogen receptor beta agonist CIDD-0149897 regulates glioma progression. Glioma stem cells (GSCs) were treated with CIDD-0149897 , and total RNA was isolated for RNA-seq analysis. Our results demonstrated that treatment with CIDD-0149897 showed upregulation of apoptotic and p53 pathways and downregulates several pathways including MYC and E2F. Overall design: Total RNA from vehicle (0.1% DMSO v/v) and CIDD-0149897 (20 µM) treated cells were isolated using RNeasy mini kit (Qiagen). Samples were prepared in triplicates and sequenced on the Illumina NovaSeq 6000 platform (Illumina Inc.) using the 150bp paired-end sequencing module.