Deep-sequencing of degraded or cleaved RNAs from barley shoot and root growing in low-Pi condition.

RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries. Total RNA isolation, PARE libraries preparation, single-reads (50 cycles) sequencing, Illumina technology, HiSeq 2500, sequencing performed at Fasteris SA (Switzerland).