Resistant and susceptible chicken lines show distinctive responses to Newcastle disease virus infection in the lung transcriptome

This study aimed to characterize differential responses between the relatively resistant (Fayoumi) and susceptible (Leghorn) chicken lines when challenged with a high titered lentogenic Newcastle Disease Virus (NDV) strain via the eyes and nostrils at three weeks of age. The lung was collected from challenged and non-challenged birds from each line at 2, 6, and 10 days-post-infection (dpi). High quality RNA was isolated, used for cDNA library construction, and sequenced on the HiSeq2500.