Global detection of high risk escape constellation F456L, R346T, K444T, F486P n=1,019,041 span 2020 - Q1 2025

ESCAPE MUTATION SUMMARY OF ANALYSIS================================================== Total sequences with ≥1 escape mutation: 1,019,041Total escape mutation events detected: 1,847,408 Mutation counts (Wuhan-Hu-1 numbering): F456L: 258,457 R346T: 760,675 K444T: 234,369 F486P: 593,907 Sequences by number of escape mutations: 1 mutation : 361,628 2 mutations: 487,325 3 mutations: 169,222 4 mutations: 866 Top 10 sequences with most escape mutations: 1. USA/MA-Broad_MGH-16621/2023 → 4 mutations 2. USA/CO-CDPHE-41673449/2024 → 4 mutations 3. USA/CA-SEARCH-233211/2024 → 4 mutations 4. Switzerland/BE-IFIK-231106_os_12/2023 → 4 mutations 5. USA/CA-CDPH-500130502/2023 → 4 mutations 6. USA/NJ-PHEL-V24000609/2024 → 4 mutations 7. USA/UT-UPHL-231221592754/2023 → 4 mutations 8. USA/CA-SEARCH-232989/2023 → 4 mutations 9. USA/CA-CDPH-500129293/2023 → 4 mutations 10. USA/SD-CDC-2-7705811/2024 → 4 mutations