C19Δ fatty acid content of wild type and CFAS mutant L. infantum and L. major promastigotes.

Derivatised fatty acid extracts from all parasites lines were analysed as described (Materials and Methods); data (mean ± SD, n = 3) are shown from (i) one clone each of wild type (+/+) L. major-LUC, +/+ L. infantum and CFAS null (ΔCFAS ::HYG/ΔCFAS ::PAC) L. infantum; (ii) four L. infantum CFAS null clones complemented by CFAS expression from either the ribosomal ([+pSSU NEO CFAS]) or endogenous ([+CFAS::NEO]) locus. Data are also included for four clones of L. major-LUC expressing CFAS from the ribosomal locus. All clone names, as used in the text, are in bold.