Figure S1 - IFN-α-Induced Upregulation of CCR5 Leads to Expanded HIV Tropism In Vivo

Gating of thymocyte subpopulations and determination of CCR5+ and Gag-p24+ thymocytes in Thy/Liv implants. After collecting 100,000 total cell events, percentages of marker-positive (CD4+, CD8+, and DP) thymocytes in the implant samples were determined by first gating on a live lymphoid cell population identified by forward- and side-scatter characteristics and then by CD3 expression. In addition, the fraction of cells positive for Gag-p24 and CCR5 was determined for all thymocyte subpopulations in each implant. W6/32-positive mean fluorescence intensity (MFI) of DP thymocytes was determined for each sample, and CD4/CD8 ratios were calculated by dividing the percentage of CD4+ cells by the percentage of CD8+ cells for each individual implant. Data shown is for HIV Ba-L-infected SCID-hu mouse #39 (Figure 1E).
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