Loss of SUV420H2-dependent chromatin compaction drives right-sided colon cancer progression [RNA-seq]

Epigenetic processes regulating gene expression contribute markedly to epithelial cell plasticity in colorectal carcinogenesis. The lysine methyltransferase SUV420H2 comprises an important regulator of epithelial plasticity and is primarily responsible for trimethylation of H4K20 (H4K20me3). Loss of H4K20me3 has been suggested as a hallmark of human cancer due to its interaction with DNMT1. However, the role of Suv4-20h2 in colorectal cancer is unknown. Molecular function of Suv4-20h2 was analysed in APT organoids. Suv4-20h2 expression was depleted in APT mouse organoids using two shrot hairpin RNAs targeting to different exons: exon4 (clone#1) and exon9 (clone#2).