miRNA signature of young, replicative and TNF-α-induced premature senescent of human microvascular endothelial cells-lung (HMVEC-L). Homo sapiens

Despite the fact that miRNAs have been extensively investigated for their involvement in diseases, senescence as well as inflammation, miRNAs involved in TNF-α- induced premature senescence remain to be uncovered. Hence, the present study aims to identify unique miRNAs and their respective signaling pathways in TNF-α-induced senescence in lung microvascular endothelial cells and novel targets for prevention or protection against premature senescence and endothelial hyperpermeability. We have employed Agilent Human MicroRNAs microarray platform to evaluate the expressions of 866 human miRNAs and 89 human viral miRNAs, based on Sanger miRNA database release 12.0. Overall design: miRNA expression profiles were established for young, replicative and TNF-α-induced premature senescent of HMVEC-L.