Supporting dataset for "Enhancing remyelination therapy with use of the bone marrow neurosphere-derived pericytes"

The dataset contained all the data for the thesis "Enhancing remyelination therapy with use of the bone marrow neurosphere-derived pericytes" This study showed a protocol to derive brain-pericyte like cells with the potential to treat demyelination from a readily accessible source, bone marrow. With the protocol modified from our lab, rat bone marrow stromal cells were used to induce bone marrow neurospheres in ultra-low adhesion conditions with a neurosphere induction medium. Afterwards, rat bone marrow neurospheres were later seeded on an adherent culture plate coated with gelatin and cultured under a pericyte induction medium to give rise to pericytes. <br>The supporting data provided the data to show the identity of the bone marrow neurosphere-derived pericytes, suggesting their subtypes and their functions. It contained the characterization of rat and human bone marrow stromal cells, neurospheres, and then the bone marrow neurosphere-derived pericytes to show their identities and high purity in each derivation stage. Furthermore, the dataset provided the statistics of the tube formation assay for rat bone marrow neurosphere-derived pericytes and also the immunocytochemistry data to show the assembly of pericytes in an endothelial network. <br>Furthermore, the dataset includes the behavioural data to show the effect of rat bone marrow neurosphere-derived pericytes on <i>in vivo </i>remyelination using shiverer model with co-transplantation with rat bone marrow neurosphere-derived oligodendrocyte precursor cells and rat bone marrow neurosphere-derived pericytes. The rotarod performance test measured the functional recovery of motor performance, and co-transplantation of rat bone marrow neurosphere-derived pericytes and rat bone marrow neurosphere-derived oligodendrocyte precursor cells was shown to improve the motor performance of the shiverer mice after 12 weeks of co-transplantation. It also includes the data from immunohistochemistry to show the presence of the transplanted cells after one week of transplantation, showing the survival of the transplanted cells.