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Proteomics of Small Extracellular Vesicles from Melanoma DCC-Derived Cell Lines

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NIAID Data Ecosystem2026-05-02 收录
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Small extracellular vesicles (sEVs) were isolated from the culture supernatant of melanoma DCC-derived cell lines (MelDCC) after 48 hours of serum-free culture. sEVs were obtained through differential ultracentrifugation, specifically as the 100K pellet, following the protocol described in detail by Théry et al. (https://doi.org/10.1002/0471143030.cb0322s30). Proteomic analysis of the isolated sEVs was performed using mass spectrometry to characterize their protein content.

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2025-02-24
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