Additional file 1 of A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones

Additional file 1: Table S1. Cytokinin (CK) concentrations (pmol mL-1) detected by HPLC-(ESI+)MS/MS in the cell-free supernatants of 46 Methylobacterium strains cultured in vitro in the DSM125 minimal medium. CK production was tested in the early stationary phase (5 – 7 days). Bacterial CKs scanned for by HPLC-(ESI+)MS/MS include: free base, riboside and nucleotide forms of Dihydrozeatin (DZ), trans-Zeatin (tZ) cis-Zeatin (cZ), and Isopentenyladenine (iP), and 2-methylthio-Zeatin (2MeSZ), 2-methylthio-Isopentenyladenine (2MeSiP), 2-methylthio-Zeatin riboside (2MeSZR), 2-methylthio-Isopentenyladenosine (MeSiPR). Values are means ± SE of 3 replicates; n.d. – not detected. Table S2. Cytokinin (CK) concentrations (pmol mL-1) detected by HPLC-(ESI+)MS/MS in the cell-free supernatants of Methylobacterium oryzae - LMG23582(T) strains cultured in vitro in the DSM125 minimal medium suplemented with various concentration of methanol. CK production was tested in 5-day old cultures. Bacterial CKs scanned for by HPLC-(ESI+)MS/MS include: free base, riboside and nucleotide forms of Dihydrozeatin (DZ), trans-Zeatin (tZ) cis-Zeatin (cZ), and Isopentenyladenine (iP), and 2-methylthio-Zeatin (2MeSZ), 2-methylthio-Isopentenyladenine (2MeSiP), 2-methylthio-Zeatin riboside (2MeSZR), 2-methylthio-Isopentenyladenosine (MeSiPR). Values are means ± SE of 3 replicates; n.d. – not detected.