Supporting data for the study "Bruceantinol targeting GPX4 triggers ferroptosis in cervical cancer"

1、BP-Table S1 Five blind connections through CB-Dock2.xlsx: Includes the input files and results for docking the BOL ligand (MOL2 format) to the GPX4 (PDB format, UniProt ID: A0A0A0MTT1), Nrf2 (PDB format, UniProt ID:AF-Q16236-F1-v4) and HO1 (PDB format, UniProt ID: AF_E7BJU4_F1_v4) protein structures. Docking was performed using CB-Dock2. The BP-Table S1 Five blind connections through CB-Dock2 file lists the binding poses and their corresponding Vina scores (in kcal/mol), where more negative scores indicate stronger predicted binding affinity.

2、Research Data-Original Western Blots.pdf: Uncropped, full-length scans of all Western blot membranes used in the study.
1)Core Mechanism Analysis (BOL Treatment):
Purpose: To demonstrate the dose-dependent effects of BOL on the expression of ferroptosis-related antioxidant proteins (Nrf2, HO-1, GPX4) and cell cycle regulators (Cyclin D1, CDK4).
Correspondence: These blots correspond to the main figures showing the downregulation of the Nrf2/HO-1/GPX4 axis (e.g., Fig. 3F, G) and cell cycle arrest (e.g., Fig. S1)mechanisms.
2)Ferroptosis Rescue Experiment (BOL + Fer-1):
Purpose: To validate that the cytotoxic effects of BOL are mediated specifically through ferroptosis. The restoration of GPX4 protein levels upon Fer-1 co-treatment supports this mechanistic claim.
Correspondence: These blots correspond to the supplementary figure (e.g., Fig. S2) illustrating the rescue of GPX4 expression by Fer-1.
3)Target Engagement Validation (CETSA for BOL-GPX4 Interaction):
Purpose: To provide biochemical evidence of direct target engagement. The increased thermal stability of GPX4 in BOL-treated samples indicates a direct binding interaction between the compound and the protein.
Correspondence: These blots correspond to the figure (e.g., Fig. 6C, D) demonstrating BOL-induced stabilization of GPX4.

3、Research Data-Analysis of DEGs in cervical cells.xlsx contains the full list of differentially expressed genes (DEGs) identified by RNA-seq in BOL-treated vs. control cells, with log2 fold change and p-values.

4、Research Data-Raw data.xlsx: This dataset provides the complete, raw numerical data underlying all figures in the associated research article investigating BOL-induced ferroptosis in cervical cancer. It contains all measurements from in vivo tumor growth, in vitro phenotypic assays (proliferation, migration, cell cycle), biochemical analyses of ferroptosis markers (ROS, iron, antioxidants), and validation experiments (GPX4 overexpression, molecular docking). Organized in a structured, worksheet-per-figure format, this collection ensures full transparency, facilitates direct verification of published results, and enables independent re-analysis or future meta-studies.