Supplementary data (BODIPY analysis, Histological scoring of liver, animal level data)

This dataset contains experimental and histological data from in vitro and in vivo models of metabolic dysfunction–associated fatty liver disease (MAFLD/NASH). It integrates quantitative cellular assays, morphometric image analysis, and animal histology scoring. The file is organized into seven sheets, each covering a distinct parameter:Bodipy_TGF-AML12 model – Quantitative analysis of lipid droplet accumulation in AML-12 cells exposed to TGF-β. Includes nuclei counts, droplet counts (≥0.5 µm²), droplet area, intensity (a.u.), and per-cell normalized values.Bodipy_PA-AML12 model – Similar Bodipy lipid droplet quantification but under palmitic acid–induced lipotoxicity. Provides droplet count, area, intensity, and per-cell normalization.NAS_score + Steatosis – Animal histology scoring of steatosis (%) and NAS scores across different treatment groups (control, MCD, MCD+THU, MCD+EW, MCD+EW+THU). Contains both raw individual data and averaged scores.Lobular inflammation – Quantification of inflammatory foci per field and corresponding NAS subscores for each animal across treatment groups.Ballooning score – Hepatocellular ballooning histology scores (0–2 scale) from control and MCD-based treatment groups.Fibrosis area – Percentage area of fibrosis from histological analysis, including raw individual values and averaged scores across conditions.AST-ALT_Hepatic TG – Biochemical markers, including hepatic triglyceride levels (mg/g liver) and liver injury enzymes (AST/ALT, depending on subsequent rows) across control and treated groups.