Topo IV reduces cohesion at both snap and non-snap loci.

(A–B) Raw cohesion values at gln (A) and dnaB (B) after Topo IV inactivation. Copy number per TetR-YFP focus was determined in parE10 (dark shaded symbols) and par+ control (light shaded symbols) cells after shift to restrictive temperature. Values are means of 3–4 experiments ±1 SD. Cells were grown to early log phase at 30°C in minimal succinate medium, shifted to 42°C, and assayed as described in Figure 1. (C) Relative gln and dnaB cohesion after Topo IV inactivation, normalized to 30°C. The difference in cohesion (copies/focus) between parE10 and WT at each time point relative to the difference at t = 0 is shown for gln and dnaB. (D) Representative micrographs of wild-type and parE10 cells at 30°C and 42°C.