Chromatin organization drives the exploration strategy of nuclear factors

The transcription factor p53 is activated in response to DNA damage. To identify the genes that are directly and indirectly regulated by p53 in the U2OS-derived DIvA cells, we performed RNAseq analysis following DNA damage. We induce DNA damage by using 4OHT, which activates the endonuclease AsiSI in this cell line leading to the formation of DNA double strand breaks. We compared p53 wild-type cells to p53 knock-out cells. RNAseq analysis of p53 WT and p53 KO DIvA cells treated with 300nM 4-OHT for 5 hours.