Identification of flagellin proteins from the citrus canker pathogens Xanthomonas citri (strain 306) and Xanthomonas aurantifolii pathotype C (strain ICMP 8435)

In this project, we have extracted the flagellum of the citrus canker pathogens Xanthomonas citri and Xanthomonas aurantifolii pathotype C and the proteins associated with these preparations were resolved by SDS-PAGE and identified by mass spectrometry. The major 50 kDa band, observed in both samples, correspond to the flagellin C (FliC) proteins. All the peptides identified by mass spectrometry shown here showed perfect matches to the X. citri and X. aurantifolii FliC proteins deposited in the GenBank (NCBI reference sequences WP_003482972 and WP_007962409, respectively).