Development of cellulosic based composites with typical three-layer orthotropic cartilage organization by combined ultrafiltration, ultrasou

The aim of this project is to develop multilayered cellulosic-based composites with structural organization pertaining to cartilage tissue engineering. Recently, frontal ultrafiltration (FU) under ultrasounds (US) processing applied on nanocrystal celluloses (CNCs) aqueous suspensions, have allowed to achieve the three orthotropic structured layers representatives of the multizonal material cartilage. In-situ SAXS revealed a parallel orientation of the CNCs near the membrane surface, an intermediate isotropic layer and a vertical orientation layer of the CNCs near the ultrasonic vibrating blade. The goal of this proposal is firstly, to freeze this structural organization thanks to the mixing of CNCS with UV cured polymer and in-situ photopolymerization applied at the end of the builded multilayered organization, secondly bring an understanding of the organization mechanism induces during FU/US/UVcuring processing from nanometric to micrometric length scales by in-situ SAXS and USAXS.