lcnRNA HOTAIR expression during adipogenesis

Adipose tissue regional distribution, rather than total body fat amount, is a key risk factor of metabolic disease. In contrast to lower-body fat accumulation which is metabolically protective, abdominal obesity correlates with increased cardiometabolic risk. The mechanisms governing adipose tissue depots intrinsic properties remain largely undefined. The long non-coding RNA HOTAIR is the top differentially expressed gene in upper-body (low expression) versus lower-body (high expression) adipose tissues. The current annotation of the HOTAIR gene is complex, with 2 predicted promoters, 4 predicted transcription start sites (TSSs), alternative splicing events and several polyadenylation sites, leading to multiple isoforms. HOTAIR transcript harbors a PRC2 binding domain at the 5 prime end, a LSD1 binding domain at the 3 prime end and several DNA binding domains. These functional domains can be truncated by usage of alternative TSS and polyadenylation sites, leading to isoforms with potentially altered functionality. To gain insight into HOTAIR function during adipogenesis, we performed long-read PacBio sequencing of captured full-length HOTAIR cDNA, providing an accurate annotation of HOTAIR isoforms expressed across adipose stem cell differentiation. CLS; Capture Long-read Sequencing. ASCs; Adipose stem cells