Isolation, characterization, proteome, miRNAome, and the embryotrophic effects of chicken egg yolk nanovesicles (vitellovesicles). Isolation, characterization, proteome, miRNAome, and the embryotrophic effects of chicken egg yolk nanovesicles (vitellovesicles)

We provide a detailed analysis of miRNA in the egg yolk vesicles. Overall design: Egg yolk of freshly oviposited commercially purchased eggs (n=3) were aseptically aspirated and diluted 10 times with phosphate-buffered saline (PBS). Egg yolk vesicles were isolated through ultracetrifugation and used for RNA extraction and sequencing.