Transcription profiling of Drosophila after exposure to microgravity in the International Space Station and in a microgravity simulator

Larvae-Pupae transition flies (Drosophila) were recovered and transport for 3 days at 12-14C to arrest development until the launch site then exposed to RT (18-20C) for some hours including the launch and trip to the International Space Station then pupae were exposed to microgravity in the ISS for 4 days and a half at 22C. Finally pupae were fixed on acetone and frozen until recovery on Earth. Four groups of samples: 1 ISS (+ground control) as described 2 RPM (microgravity simulator on Earth) as described 3 RPM without constrains (No MAMBA container and only 5 days exposure without cold transport) and 4 centrifuge 10g without constrains control.

在12-14℃的条件下，对果蝇幼虫至蛹的过渡阶段进行了复苏与运输，持续3日，以延缓其发育直至发射地点。随后，将蛹暴露于室温（18-20℃）数小时，包括发射过程及前往国际空间站的路程。在国际空间站内，蛹在22℃的微重力环境下暴露了4天半。最终，蛹在乙醚中固定并冷冻，直至地球上的复苏。实验分为四组样本：1）国际空间站组（包括地面对照组）如描述所述；2）地球上的微重力模拟器RPM组如描述所述；3）RPM组未施加限制（无MAMBA容器，仅暴露5天，无低温运输）；4）离心机10g未施加限制的对照组。