The Hxk1 protein of Candida albicans influences gene expression of both metabolic and virulence related genes. The Hxk1 protein of Candida albicans influences gene expression of both metabolic and virulence related genes

The Hxk1 protein of Candida albicans is a moonlighting protein with a wide variety of functions. It phosphorylates N-acetylglucosamine (GlcNAc) which is necessary for different purposes in the cell including energy and chitin production. Further, this protein is a regulator for morphogenesis independent of its function during GlcNAc catabolism.To identify the regulatory role of Hxk1 and putative downstream regulator Hxk2, we performed CUT&RUN experiments for GFP-tagged Hxk1 strains and GFP-tagged Hxk2 strains. Overall design: CUT&RUN for 3 biological replicates of GFP-tagged Hxk1, GFP-tagged Hxk2 and corresponding IgG negative controls.