Next Generation Sequencing Transcriptome Quantitative Analysis of Placenta of at embryonic day 19

Obesity during pregnancy is associated with fetal growth restriction in the offspring. We used RNA next generation sequencing analysis (HiSeq2000) to have a snapshot of the placenta transcriptome at embryonic day 19 to examine whether mice exposed to maternal obesity had significant changes in their transcriptome which may lead to growth restriction in the fetus. Overall design: C57BL/6J mice were fed either a standard control diet, RM1 [7% simple carbohydrates/3% lipids (w/w)] or an energy-rich obesogenic diet [10% simple carbohydrates/20% lipids (w/w)] plus sweetened condensed milk [55% simple carbohydrates, 8% lipids, 8% protein (w/w), Nestle (Nestle, UK), supplemented with mineral and vitamin mix AIN93G]. Female mice were mated, and Pregnant mice were housed individually and maintained on their respective diets until sacrafice. A subset of the mice on the Obesegenic diet were exercised until E17 of pregnancy. Placenta tissue was collected at embryonic day 19.