Gene function rather than reproductive mode drives the evolution of RNA helicases in sexual and apomictic Boechera

In higher plants sexual and asexual reproduction through seeds (apomixis) have evolved as alternative strategies. Evolutionary advantages leading to coexistence of both reproductive modes are currently not well understood. It is expected that accumulation of deleterious mutations leads to a rapid elimination of apomictic lineages from populations. In this line, apomixis originated repeatedly, likely from deregulation of the sexual pathway, leading to alterations in the development of reproductive lineages (germlines) in apomicts as compared to sexual plants. This potentially involves mutations in genes controlling reproduction.

Increasing evidence suggests that RNA helicases are crucial regulators of germline development. To gain insights into the evolution of 58 members of this diverse gene family in sexual and apomictic plants, we applied target enrichment combined with Next Generation Sequencing to identify allelic variants from 24 accessions of the genus Boechera, comprising sexu...