Gene signature of young, replicative and TNF-α-induced premature senescent of human microvascular endothelial cells-lung (HMVEC-L)

Despite the fact that miRNAs have been extensively investigated for their involvement in diseases, senescence as well as inflammation, miRNAs involved in TNF-α- induced premature senescence remain to be uncovered. Hence, the present study aims to identify unique miRNAs and their respective signaling pathways in TNF-α-induced senescence in lung microvascular endothelial cells and novel targets for prevention or protection against premature senescence and endothelial hyperpermeability. Gene profiling was established using the same RNA input as that used for miRNA profiing. We have employed Agilent Whole Human Genome microarray platform to evaluate the expressions of 19,596 human genes. Gene expression profiles were established for young, replicative and TNF-α-induced premature senescent of HMVEC-L.