Genome wide analysis of the changes in gene expression following co-culture of A431 cancer cells and stromal fibroblasts

This experiment tested the hypothesis that distinct changes in gene expression are triggered in response to direct contact between cancer cells and fibroblasts, as opposed to co-cultures in which the cells are physically separated by membrane that permits diffusion A431-GFP or VCAF2b-mCherry were cultured in either mono-cultures, co-cultures separated by a 0.4micron filter, or direct co-cultures followed by FACS purification.