Benchmarking metagenomic extraction protocols for culture-independent Nanopore sequencing

This study aimed to characterize six extraction kits based on their ability to extract DNA from metagenomic samples and the sequence data generated from these DNA extracts with Oxford Nanopore Technologies' long-read sequencing. Extraction kits evaluated include: Norgen Stool DNA Isolation, ZymoBIOMICS Quick-DNA HMW MagBead Kit (ZymoMag), Qiagen DNeasy Blood and Tissue, MN NucleoMag DNA Microbiome, ZymoBIOMICS DNA Mini Prep (ZymoMini), and Qiagen DNeasy PowerSoil/QIAamp PowerFecal Pro Kit. Kits were tested by extracting DNA from the ZymoBIOMICS Microbial Community Standard.