Liver Transcriptomics from Male and Female Mice of Three Strains on Control, Medium Protein and Low Protein Diets

Mice were put on either a control, medium or low protein diet and their liver transcriptomic expression was measured after 13 weeks on diet after an overnight fast. This was to discover the impact of sex, strain and protein level on the metabolism of the liver. Overall design: To compare the metabolic effect of a Low and Medium Protein Diets on different sexes and strains, 10-week-old mice were placed on either a Control, Medium Protein or Low Protein diets for 13 weeks. All mice were maintained at a temperature of approximately 22°C. Mice were euthanized by cervical dislocation and livers were harvested and snap frozen in liquid nitrogen following overnight (16-hr) fasting.