DataSheet_8_Genome-Wide Identification of Dickeya solani Transcriptional Units Up-Regulated in Response to Plant Tissues From a Crop-Host Solanum tuberosum and a Weed-Host Solanum dulcamara.fasta

Dickeya solani is a Gram-negative bacterium able to cause disease symptoms on a variety of crop and ornamental plants worldwide. Weeds including Solanum dulcamara (bittersweet nightshade) growing near agricultural fields have been reported to support populations of soft rot bacteria in natural settings. However, little is known about the specific interaction of D. solani with such weed plants that may contribute to its success as an agricultural pathogen. The aim of this work was to assess the interaction of D. solani with its crop plant (Solanum tuberosum) and an alternative (S. dulcamara) host plant. From a collection of 10,000 Tn5 transposon mutants of D. solani IPO2222 carrying an inducible, promotorless gusA reporter gene, 210 were identified that exhibited plant tissue-dependent expression of the gene/operon into which the Tn5 insertion had occurred. Thirteen Tn5 mutants exhibiting the greatest plant tissue induction of such transcriptional units in S. tuberosum or S. dulcamara as measured by qRT-PCR were assessed for plant host colonization, virulence, and ability to macerate plant tissue, as well as phenotypes likely to contribute to the ecological fitness of D. solani, including growth rate, carbon and nitrogen source utilization, motility, chemotaxis toward plant extracts, biofilm formation, growth under anaerobic conditions and quorum sensing. These 13 transcriptional units encode proteins involved in bacterial interactions with plants, with functions linked to cell envelope structure, chemotaxis and carbon metabolism. The selected 13 genes/operons were differentially expressed in, and thus contributed preferentially to D. solani fitness in potato and/or S. dulcamara stem, leaf, and root tissues.

狄氏菌（Dickeya solani）是一种革兰氏阴性细菌，能够在全球范围内引发多种作物和观赏性植物病害症状。据报道，生长在农田附近的杂草，如茄属植物（Solanum dulcamara，即苦甜夜shade），能够在自然环境中支持软腐细菌群体的生长。然而，关于狄氏菌与这类杂草之间的特定相互作用，以及这种相互作用可能对其作为农业病原体的成功所起到的作用，了解甚少。本研究的目的是评估狄氏菌与其作物宿主（马铃薯Solanum tuberosum）以及替代宿主植物（S. dulcamara）之间的相互作用。通过对10,000个携带诱导型、无启动子gusA报告基因的Tn5转座子突变体狄氏菌IPO2222的收集，其中210个表现出Tn5插入位点的基因/操纵子对植物组织的依赖性表达。通过qRT-PCR测定，在马铃薯和S. dulcamara中，13个突变体显示出最大程度的植物组织转录单位诱导。这13个突变体在植物宿主定殖、致病性、破坏植物组织的能力以及可能有助于狄氏菌生态适应性的表型方面进行了评估，包括生长速率、碳和氮源利用、运动性、对植物提取物的趋化性、生物膜形成、无氧条件下的生长和群体感应。这些转录单位编码的蛋白质参与细菌与植物的相互作用，其功能与细胞壁结构、趋化性和碳代谢相关。所选的13个基因/操纵子在马铃薯和/或S. dulcamara的茎、叶和根组织中差异表达，从而优先贡献于狄氏菌的生态适应性。