Transcript profiling of wrinkled1 mutant embryos in Arabidopsis thaliana

Understanding the mechanisms of transcriptional regulation for genes involved with FA biosynthesis and TAG accumulation in seeds can represent an important step in the development of crops with increased oil content. Among well studied transcription factors (TFs), WRINKLED1 (WRI1), a member of the APETALA2 (AP2) family of TFs, has been characterized as a central regulator of FA biosynthesis in seeds of many plant species. Despite its importance for the process, the detailed mechanisms of WRI1 function in regulating FA biosynthesis in seeds is still not well understood. This study aimed to characterize the gene networks controled by WRI1 during soybean embryo development and its function as central regulator of FA biosynthesis and TAG accumulation in seeds. In this part of the study, we identified genes misregulated in the embryos of the Arabidopsis mutant wrinkled1-1, when compared to wild-type. Embryos from homozygous wrinkled1-1 or wild-type plants were collected, 8 days after pollination. Three biological replicates per genotype were analyzed.