We performed hnRNPM iCLIP to identify the potential targets. Briefly, 5,000,000 AGS cells expressing FLAG-hnRNPM were ultraviolet crosslinked to a total of 0.4 J/cm2 in a UV cross-linker and harvested
We used GFP-tagged SR proteins expressed at endogenous levels and iCLIP to compare the extent and pattern of SR protein binding to expression-matched lincRNAs and protein-coding RNAs For each GFP-tagg
This experiment aims to globally identify LARP6 binding sites on the transcriptome by performing iCLIP in MDA-MB231 cell lines expressing GFP-LARP6. MDA-MB231 cell lines expressing GFP only were used
This experiment identifies hnRNP A1 binding sites transcriptome-wide in Hela cells. HeLa cells with inducible expression of T7-tagged hnRNP A1 were grown to approximately 90% confluence and then subje
